Kinetics aspects of Gamma-hydroxybutyrate dehydrogenase

3-hydroxybutyrate dehydrogenase

The enzymic determination of D-3-hydroxybutyrate and acetoacetate normally involves the use of 3hydroxybutyrate dehydrogenase (HBDH, EC 1.1.1.30) of bacterial origin. We show that HBDH from Rhodopseudomonas spheroides (BCL, grade II) contains a 3-hydroxyisobutyrate dehydrogenase (HIBDH) activity: activity with 3-hydroxyisobutyrate as substrate was > 10% of that with 3-hydroxybutyrate. However, ...

Acute Gamma Hydroxybutyrate Toxicity

Gamma-hydroxybutyrate / Butyric Acid

Gamma-hydroxybutyrate / butyric acid, ambiguously called GHB, presents some unique challenges for analysis due in part to its acidity, high polarity, and high solubility in aqueous solution. Its chemistry is complicated by its conversion into the corresponding lactone compound, where the GHB molecule condenses to form a cyclic ester with a five-membered ring. This compound, gamma-butyrolactone ...

Gamma-Hydroxybutyrate (GHB) Withdrawal

Gamma-hydroxybutyrate, gamma-butyrolactone, and 1,4-butanediol.

1 National Poisons Centre, Department of Preventive and Social Medicine, University of Otago, Dunedin, New Zealand 2 Department of Anesthesia and Intensive Care Medicine, Surgical Sciences, Blå Stråket 5, Sahlgrenska University Hospital, Gothenburg, Sweden 3 National Poisons Information Service (Birmingham Unit) and West Midlands Poisons Unit, City Hospital, Birmingham, UK; School of Bioscience...